Cell cocultures on coated scaffolds applied to liver models
by Odin Ramírez-Fernández; Rafael Godínez; Esmeralda Zuñiga-Aguilar; Luis E. Gómez-Quiroz; María C. Gutiérrez-Ruiz; Juan Morales; Roberto Olayo
International Journal of Medical Engineering and Informatics (IJMEI), Vol. 9, No. 4, 2017

Abstract: Volumetric cell cultures are widely used as an experimental model to mimic the characteristics and responses of cell in tissues in vivo. Hepatocytes, particularly, are highly specialised cells, their geometrical conformation, and their coculture with no parenquimal cells and vascularised tissue, are essential to their physiology and proper function. Polymeric scaffolds for cell growth have been modified through plasma polymerisation to improve their biological properties. This work presents a Poly (L-lactic acid) (PLLA) coated scaffold with polypyrrole plasma doped with iodine (Ppy-I). The scaffold where seeded with two different cell lines, hepatocytes (HepG2) with stellate cells (Ito), and hepatocytes (HepG2) with endothelial cells (HUVEC), the data were evaluated against control hepatocytes (HepG2) culture. The morphology of the cells was evaluated by microscopy; to corroborate the cell proliferation on the material, images of the samples with the cells were taking three times per week. Total protein in the supernatant was measured, and showed that in the endothelial coculture increased 25% the protein production and the stellate coculture increased 20% the protein production against the hepatocyte culture. In conclusion, we obtain a Ppy-I surface coculture that optimises the conditions for better liver models.

Online publication date: Mon, 02-Oct-2017

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Medical Engineering and Informatics (IJMEI):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com