SWATH-MS in proteomics: current status
by Jing Wang; Quanhu Sheng; Yu Shyr
International Journal of Computational Biology and Drug Design (IJCBDD), Vol. 8, No. 3, 2015

Abstract: Sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) is a new strategy for identification and quantification of proteins and proteomes proposed in 2012. SWATH-MS operates in unbiased data-independent acquisition (DIA) mode and combines the advantages of two widely used MS-based techniques: shotgun (high throughput) and SRM proteomics (high sensitivity and reproducibility). In this review, we provide an introduction of the SWATH-MS method, its application, and a perspective on the future of this powerful technology.

Online publication date: Wed, 30-Sep-2015

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