The investigation of novel glucose oxidase biosensor based on graphene-clay nanocomposite Online publication date: Fri, 07-Nov-2014
by Khadijeh Eskandari; Farideh Gouranlou; Mehdi Kamali; Ali Morsali
International Journal of Biomedical Nanoscience and Nanotechnology (IJBNN), Vol. 3, No. 3, 2014
Abstract: A feasible and fast method to fabricate glucose biosensor was developed by immobilisation of glucose oxidase (GOx) to nano-sepiolite-graphene nanocomposite/glassy carbon electrode. The direct electrochemistry of glucose oxidase not containing medioter and worked in air saturated condition for similarity of in vivo usage. The nano-sepiolite-graphene nanocomposite shows a high conductive and biocompatible template for enzyme immobilisation. The immobilised GOx on nanocomposite shows a couple of well defined reversible peak with the anodic and cathodic peak potentials of -430 and -460 mV (vs. Ag/AgCl), corresponding to the FAD/FADH2 redox couple, and with a formal potential of -445 mV in 0.1 M phosphate buffer solution at pH = 7. The linear concentration rang of biosensor is 0.14-3.14 µM, and with detection limit of 0.04 µM. Also, the life time of biosensor is more than four months.
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