Detection of biomarker p53 mutated gene by a silicon nanowire nanosensor Online publication date: Fri, 05-Apr-2013
by Van Binh Pham; Hien Le Thi; Xuan Thanh Tung Pham; Van Vinh To; Ngoc Thuy Duong Dang; Thi Thanh Tuyen Le; Viet Long Nguyen; Van Thao Le; Truong Giang Bui; Mau Chien Dang; Duy Hien Tong
International Journal of Nanotechnology (IJNT), Vol. 10, No. 3/4, 2013
Abstract: Among biosensors of various types, the silicon nanowire field-effect transistor (SiNW-FET) is believed to be the most sensitive and powerful device for bio-applications. In fact, these devices have demonstrated the ability to detect a variety of analyses such as particular DNA sequences, cancer biomarkers and larger entities such as viruses. The principle of sensing is based on the variation of conductivity resulting from a disturbance of charge on the surface of the SiNW-FET. In this study, we have used two different processes for surface modification of silicon nanowire. As the DNA of liver cancer cells has mutation at codon 249 which changes one base guanine (G) by base thymine (T), we thus designed 17-mer DNA oligonucleotides, which were used as a receptor to capture the DNA of p53 mutated gene. The fabricated SiNW biosensor could detect DNA of p53 mutated gene with concentrations down to about 100 pM.
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email subs@inderscience.com
Our Blog 
Follow us on Twitter 
Visit us on Facebook