Low doses of γ-radiation induce consistent protein expression changes in human leukocytes Online publication date: Sat, 07-Feb-2015
by Sara Skiöld; Susanne Becker; Ulf Hellman; Gert Auer; Ingemar Näslund; Mats Harms-Ringdahl; Siamak Haghdoost
International Journal of Low Radiation (IJLR), Vol. 8, No. 5/6, 2011
Abstract: Twenty percent of cancer patients experience adverse effects after radiotherapy. The therapeutic doses are adjusted to the most sensitive individuals, resulting in a suboptimal dose for many patients. At present there is no screening system available to predict individual radiosensitivity. The main aim of this study is to investigate differences in protein expression pathways induced by low doses of radiation in whole blood obtained from radiosensitive and non-radiosensitive patients. To address this aim, a protocol for exposure condition, dose and analysis of whole blood was developed. The conditions for handling blood samples were optimised. The optimal doses and post irradiation conditions were determined. We found that 1 mGy and 150 mGy significantly changed protein expression in leukocytes collected from the two donors. The result shows that the protocol developed is suitable for characterisation of proteomic profiles associated with low dose exposure of leukocytes.
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Low Radiation (IJLR):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email subs@inderscience.com
Our Blog 
Follow us on Twitter 
Visit us on Facebook