Efficacy of selected probiotic cultures to inhibit Enterobacter sakazakii ATCC 12868 in model reconstituted dairy products
by Makuba A. Lihono; Aubrey F. Mendonca; Bledar Bisha; La Tanya Bankston; Terri D. Boylston
International Journal of Food Safety, Nutrition and Public Health (IJFSNPH), Vol. 4, No. 2/3/4, 2011

Abstract: Enterobacter sakazakii ATCC 12868 (ES) is a very dangerous opportunistic organism likely to contaminate post-pasteurised dry milk products with a high mortality rate (40-80%). This study evaluated the antimicrobial efficacy of probiotics against ES in reconstituted dried infant formula. Initial study at 30C showed that Enterococcus faecium M-74 (EF) to exhibit the strongest inhibition when compared with Lactobacillus acidophilus 74-2 (LA) and Pediococcus acidilacticii (PA). In later study, initial populations of ES and EF were ~10² CFU/ml and 10⁹ CFU/ml in infant formula, respectively. At 12h, ES in controls at 30 and 35C increased to 6.54 and 7.95 log10 CFU/ml, respectively. At 12 hours, ES in formula (30C) with EF decreased to 0.52 log10 CFU/ml and was undetected (< 1.0 CFU/ml) at 35C. EF rapidly reduce the pH of the infant formula below pH 5.0 in the first four hours of fermentation at 35C, more than at 30C. The inhibitory effect of EF may be due to the acidification of the formula.

Online publication date: Thu, 27-Nov-2014

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