Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach
by Aurelie Couesnon, Cesare Colasante, Jordi Molgo, Michel R. Popoff
The Botulinum J. (TBJ), Vol. 1, No. 4, 2010

Abstract: Recombinant C-terminus (Hc) of botulinum neurotoxin A (BoNT/A) was used to monitor toxin entry into NG108-15 neuronal cells and m-IC_cl2 intestinal cells by both fluorescence and electronic microscopy. BoNT/A Hc entered neuronal cells via a clathrin pathway and intestinal cells mainly via non-clathrin vesicles. Upon incubation at 4°C, BoNT/A Hc gold particles were mainly distributed to neuronal cell periphery and to a lower extent to m-IC_cl2 cells. After incubation at 37°C, BoNT/A Hc mostly transited in coated vesicles in NG108-15 cells and in uncoated vesicles of m-IC_cl2 cells, which support the transcytotic passage of the toxin.

Online publication date: Thu, 17-Mar-2011

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the The Botulinum J. (TBJ):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com