Lipid rafts: inhibitors of endocytosis of botulinum neurotoxin Online publication date: Thu, 17-Mar-2011
by Kimberly A. Petro, Cara-Lynne Schengrund
The Botulinum J. (TBJ), Vol. 1, No. 4, 2010
Abstract: Methyl-ß-cyclodextrin (MßCD) was used to extract cholesterol from plasma membranes of N2a murine neuroblastoma cells. MßCD-disruption of lipid rafts enhanced cleavage of SNAP-25 by BoNT/A, axonogenesis, expression of gangliosides GM1 and GD1a, association of GM1 with the nucleus, and exo- and endo-cytosis. Analysis of endocytosis of an anti-synaptotagmin I monoclonal antibody that recognised part of the protein found on the luminal surface of synaptic vesicles indicated that it was taken up more readily by MßCD-treated cells, supporting the hypothesis that enhanced exocytosis of synaptic vesicles provided more SV2 on the cell surface thereby making more available for toxin binding.
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