Protein-loaded chitosan nanoparticles modulate uptake and antigen presentation of hen egg-white lysozyme by murine peritoneal macrophages
by S. Madrigal-Carballo, M. Esquivel, M. Sibaja, J. Vega-Baudrit
International Journal of Nanoparticles (IJNP), Vol. 3, No. 2, 2010

Abstract: Chitosan binds to negatively charged tripolyphosphate (TPP) by an electrostatic interaction driven by its positively charged amino group. This interaction allows developing stable nanoparticles suitable as a carrier and controlled release system for drugs and vaccines. We study the effect of chitosan nanoparticles (CNp) on the uptake and antigen presentation of the model protein hen-egg white lysozyme (HEL) to peritoneal macrophages isolated from mice. Results showed that after four hours of pre-incubation with a T-cell hybridoma line cocultured with murine peritoneal macrophages, only trace amounts of IL-2 were detected in treatments with HEL alone, whereas cocultures treated with HEL-CNp had already reached maximum IL-2 expression. Confocal microscopy studies showed that CNp had a higher uptake rate of the fluorescently labelled protein than the protein itself after 30 min of incubation with the peritoneal macrophages. Our results suggest that CNp system is a potential candidate for an oral vaccine delivery system.

Online publication date: Wed, 25-Aug-2010

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