Studies on cloning and economic production of recombinant Streptokinase in a novel osmotically inducible E. Coli GJ1158
by K.K. Pulicherla, A. Krishna Satya, V.P.B. Rekha, G.S. Gadupudi, J.B. Peravali, K.R.S. Sambasiva Rao
International Journal of Biomedical Engineering and Technology (IJBET), Vol. 4, No. 1, 2010

Abstract: Streptokinase, a 47KDa protein is efficaciously used as a thrombolytic drug. Though it is relatively inexpensive, being a bacterial protein, Streptokinase is antigenic to humans. The low yields obtained in Streptokinase production, and the pathogenic nature of its natural host is the principal reasons to search for a recombinant producer. The report includes successful isolation of the Streptokinase gene from the wild host Streptococcus species MTCC389 and transforming it to E. coli expression hosts BL21(DE3) and GJ1158 through pRSET-A plasmid cloning vector, optimisation of media and analysis of successful expression of recombinant streptokinase in new host.

Online publication date: Sat, 31-Jul-2010

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