Evaluation of radioinduced damage and repair capacity in human breast cancer cells, MCF-7 and T-47D
by Flavia Gomes Silva Val Valgode, Carlos Roberto Jorge Soares, Paolo Bartolini, Kayo Okazaki
International Journal of Low Radiation (IJLR), Vol. 6, No. 4, 2009

Abstract: Ionising radiation is an established etiological agent for breast cancer, but is also a therapeutic modality used in cancer treatment. The accumulation of deoxyribonucleic acid (DNA) damage and deficient DNA repair are considered susceptibility factors that predispose individuals to breast cancer development. In the present study, the genetic damage induced by gamma radiation and repair capacity in the target cells, i.e., cell lines originating from breast cancer, were analysed using a micronucleus (MN) test and comet assay (single-cell alkaline gel electrophoresis). Two breast tumour cell lines, MCF-7 and T-47D, were irradiated in a ⁶⁰Co source (0.722 Gy/min) with various doses (0.5, 1.0, 2.0, 4.0 and 5.0 Gy). Cytogenetic data showed similar spontaneous damage of two cell lines; the radioinduced damage, however, was higher in T-47D starting from 2 Gy, with a more accelerated proliferation rate than MCF-7 at all analysed doses. Both tumour cell lines were able to repair a considerable part of the radioinduced damage within 1 h after exposure, indicating a relative radioresistance of these cell lines to the genotoxic action of ionising radiation.

Online publication date: Wed, 18-Nov-2009

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