Nanogenomics for medicine: emerging strategies to overcome bottlenecks in siRNA-based therapy of human diseases
by Tariq Alsheddi, Alexandre Vetcher, Ancha Baranova
International Journal of Nano and Biomaterials (IJNBM), Vol. 2, No. 1/2/3/4/5, 2009

Abstract: Silencing of gene expression by siRNA holds a promise both for a drug target discovery and as a therapy. Major bottlenecks of siRNA technology include 'off-target' silencing effects and problems with siRNA degradation before delivery. We propose a two-fold approach aimed at curtailing described shortcomings. First, before pre-clinical tests each siRNA shall be matched to comprehensive redundancy minimiser (CRM) curated database that contain comprehensively mapped all unique sequences ('targets') 9 nt to 15 nt in size within human transcriptome. Second, we propose to deliver siRNA within DNA-wrapped RNA-containing nanoparticle formed by partial dsRNA-DNA triplex. The DNA core will be chemically modified to promote triplex formation/folding. In these binary nanoparticles, DNA will serve as the biodegradable 'basket' that could be custom designed in order to enhance its interaction with the particular siRNA duplex. We also propose to covalently bind DNA basket to targeting peptides ensuring addressed delivery of the therapeutic nanoparticle.

Online publication date: Sat, 08-Aug-2009

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nano and Biomaterials (IJNBM):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com