Reduction of aflatoxin B₁ and Ochratoxin A in cocoa beans infected with Aspergillus via Ergosterol Value
by S.O. Aroyeun, G.O. Adegoke, J. Varga, J. Teren
World Review of Science, Technology and Sustainable Development (WRSTSD), Vol. 6, No. 1, 2009

Abstract: Aflatoxigenic and ochratoxigenic isolates were used to contaminate cocoa beans in this study and Ergosteol Values (EV) were assessed by means of visual inspection, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) and Gas Chromatography–Mass Spectrometer (GC–MS), respectively. Different concentrations of Aframomum danielli essential oils at 500, 1,000, 1,500, and 2,000 ppm were used to treat the infected samples. It was found that aflatoxin B1 (afB₁) and Ochratoxin A (OTA) correlated with EV and all correlated with cocoa bean infection and positively r = 0.80 and 0.79. Toxic afB₁ in the Aspergillus flavus contaminated cocoa beans reduced from 1.4 ng g10⁻¹ at 1,000 ppm essential oil and reached 0.08 ng g10⁻¹ at 2,000 ppm. The afB1 reduction efficiency with A. danielli was found to be 94.3% while ergosterol reduced by 78.5%. The OTA and the EV of other cocoa bean sample varied according to the contaminating species and its OTA production capacity. The active components of A. danielli such as the mono-terpenes, alkaloids and phenolic acids in the essential oils might have been responsible for the reduction in afB₁, OTA and EV as evidenced in this study.

Online publication date: Wed, 07-Jan-2009

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the World Review of Science, Technology and Sustainable Development (WRSTSD):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com