Computationally Optimised DNA Assembly of synthetic genes
by Liza S.Z. Larsen, Christopher D. Wassman, G. Wesley Hatfield, Richard H. Lathrop
International Journal of Bioinformatics Research and Applications (IJBRA), Vol. 4, No. 3, 2008

Abstract: Gene synthesis is hampered by two obstacles: improper assembly of oligonucleotides; oligonucleotide defects incurred during chemical synthesis. To overcome the first problem, we describe the employment of a Computationally Optimised DNA Assembly (CODA) algorithm that uses the degeneracy of the genetic code to design overlapping oligonucleotides with thermodynamic properties for self-assembly into a single, linear, DNA product. To address the second problem, we describe a hierarchical assembly strategy that reduces the incorporation of defective oligonucleotides into full-length gene constructs. The CODA algorithm and these biological methods enable fast, simple and reliable assemblies of sequence-correct full-length genes.

Online publication date: Thu, 17-Jul-2008

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