The role of genomic DNA and introns in gene transfer Online publication date: Sun, 13-Jul-2003
by Assem Mohamed M. Ali
International Journal of Biotechnology (IJBT), Vol. 3, No. 3/4, 2001
Abstract: This paper deals with the advantages of using genomic DNA from developed organs in gene transfer and the production of transgenic organisms. It also discusses the role of introns, the functional genes and how they are integrated into the host genomic. The integrated unfunctional DNA never causes defects or deleterious effects but on the contrary it causes repair and heterosis. The utilisation of vectors especially viruses are completely avoided.
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