Genotoxic evaluation of [DOTA, Tyr³]octreotate labelled with ¹³¹I and ¹⁷⁷Lu in human peripheral lymphocytes in vitro by micronucleus assay
by Miriam Fussae Suzuki, Marcia Augusta Da Silva, Jose De Souza Caldeira Filho, Maria Tereza Colturato, Elaine Bortoleti de Araujo, Paolo Bartolini, Kayo Okazaki
International Journal of Low Radiation (IJLR), Vol. 4, No. 2, 2007

Abstract: [DOTA, Tyr³]octreotate has been used for cancer diagnosis and therapy because of its high affinity to somatostatin subtype receptors sstr₂ and sstr₅. The aim of this study was to evaluate the cytogenetic effect of radio-labelled [DOTA, Tyr³]octreotate in blood cells in vitro, using the cytokinesis-block micronucleus assay. Blood samples of healthy donors were exposed to different activities of [DOTA, Tyr³]octreotate labelled with ¹³¹I (n = 3) and ¹⁷⁷Lu (n = 3), where radioactive concentration ranged from 600 to 5600 kBq/mL. The cells were cultivated according to criteria adopted by the IAEA (Vienna). The results showed a positive correlation between radioactive concentrations (X) and the frequency of binucleated cells with micronuclei (Y) (P < 0.05). The linear equations were similar: for the ¹³¹I labelled, Y = (1.634 ± 0.236) = (0.912 ± 0.137) 10–3 X and for the ¹⁷⁷Lu labelled, Y = (1.715 ± 0.342) = (0.743 ± 0.135) 10–3 X.

Online publication date: Wed, 17-Oct-2007

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