Research on gene therapy of neurons based on narcotic nanoparticles intervention in cerebral ischemia Online publication date: Mon, 13-Feb-2023
by Fan Zhang; Fan Lei; Xing-peng Xiao
International Journal of Nanotechnology (IJNT), Vol. 19, No. 6/7/8/9/10/11, 2022
Abstract: Objective: To detect the efficiency of gene therapy of anaesthetic nanoparticle drug materials β-CD-PAMAM and β-CD-PAMAM/pEGFP-N3 in vitro to intervene in cerebral ischemic neurons, in preparation for further in vivo gene therapy. Methods: Standard non-viral vector polyamidoamine dendrimer PAMAM was used as a positive control group. Results: Cytotoxicity test: In the two types of cells, when N/P<10, all anaesthetic nanoparticle drug material carriers and their complexes showed low toxicity, and the cell survival rate was above 80% (P<0.05); When N/P>20, the cell survival rate of β-CDPAMAM and β-CD-PAMAM/pEGFP-N3 complex was higher than that of PAMAM and PAMAM/pEGFP-N3 complex (P < 0.05). Conclusion: The cytotoxicity and gene transfection efficiency of β-CD-PAMAM have been clarified, and it has been confirmed that β-CD-PAMAM can be used as a carrier for gene transfer in Hek293 and SH-SY5Y cells, and can further assist with gene therapy of cerebral ischemic neurons.
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email subs@inderscience.com
Our Blog 
Follow us on Twitter 
Visit us on Facebook