Preparation of nano biosensors for detection of intestinal flora and cellulose molecular weight
by Xiaoqian Liu
International Journal of Nanotechnology (IJNT), Vol. 19, No. 6/7/8/9/10/11, 2022

Abstract: Fe₂O₃@Au and DNA-loaded NPs were prepared first, which were then attached to GCE to prepare Fe₂O₃@Au/GCE biosensor. The influence of different polymerisation temperature, cellulose molecule solutions of different concentrations, cellulose molecule binding time, and electrolyte concentration on the sensor was measured. The results showed that the diffraction peaks of Fe₂O₃ NPs appeared at 18°, 22°, 33°, 47°, and 54°, respectively, corresponding to the bitplanes of (222), (312), (415), (524), (425) of Fe₂O₃ NPs. The biosensor had a potential peak around 0.9V at different polymerisation temperatures, and the potential peak intensity results were expressed as 40°C<45°C<60°C<55°C<50°C. The binding capacity of the biosensor to the cellulose molecular weight in different ratios were expressed as 1:3>1:5>1:4>1:2. The relative standard error of the peak current by the biosensor in 1.5 mM Fe(CN)₆^3-/4- solution was 1.21%. Fe₂O₃@Au/GCE biosensor can effectively detect intestinal flora and determine the cellulose molecular weight, with high selectivity, sensitivity, and reproducibility.

Online publication date: Mon, 13-Feb-2023

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