A strategy for Ordered single molecule Sequencing based on Nanomanipulation (OsmSN)
by Yi Zhang, Junhong Lu, Minqian Li, Jun Hu
International Journal of Nanotechnology (IJNT), Vol. 4, No. 1/2, 2007

Abstract: In this mini-review we present the concept and the advancement of a novel strategy termed ''ordered single molecule sequencing based on nanomanipulation (OsmSN)'' for whole-genome sequencing. Techniques have been developed to integrate the processes including dissection and isolation of DNA molecules with sufficient spatial resolution together with the subsequent single molecule PCR to achieve the analysis of a particular DNA fragment from a genome DNA molecule. A nanoparticle-assisted PCR protocol was also developed to enhance the specificity and yield of PCR to meet the special requirement of the OsmSN.

Online publication date: Mon, 05-Feb-2007

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com