Dynamic basis of mRNA processing in male germline stem cell pluripotency and reprogramming by alternative polyadenylation Online publication date: Fri, 13-May-2022
by Praveen Kumar Guttula; Mukesh Kumar Gupta
International Journal of Data Mining and Bioinformatics (IJDMB), Vol. 25, No. 3/4, 2021
Abstract: Male Germline Stem (GS) cells are responsible for sperm production in males throughout the adulthood. Under in vitro culture conditions, these cells can undergo the reprogramming to generate Germline Pluripotent Stem (GPS) cells. However, the mechanism of reprogramming is elusive. The Alternative Polyadenylation (APA) pattern in GS and GPS cells was identified using APA Detect and 3' Untranslated Regions (3'-UTR) length was analysed in differentially expressed mRNAs. Results showed that, APA events regulated the expression of 78 genes and caused the shortening of 3'-UTR during the reprogramming. The APA regulated genes were found to be involved in the regulation of mRNA processing and splicing with shortened 3'-UTR. 28 miRNAs were found to target the APA-regulated genes. These results suggest that APA events might coordinate the reprogramming of GS cells to GPS cells by dynamic processing of 3'-UTR length in differentially expressed mRNAs through miRNAs.
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