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Antibacterial potential of silver nanoparticles synthesised by electron beam irradiation
by Rani M. Pattabi, Kandikere R. Sridhar, Srinath Gopakumar, Bhat Vinayachandra, Manjunatha Pattabi
International Journal of Nanoparticles (IJNP), Vol. 3, No. 1, 2010

 

Abstract: Antibacterial activity of silver nanoparticles synthesised by irradiating an aqueous solution of AgNO3 in a bio-compatible polymer polyvinyl alcohol (PVA) with electrons from a microtron, at doses of 1 kGy and 2 kGy, has been evaluated against selected Gram-positive and Gram-negative bacteria. Nanoparticles produced by electron beam irradiation (EBI) at 1 kGy showed significantly higher inhibitory effects than 2 kGy on Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Streptococcus pneumoniae). The inhibition was highest on P. aeruginosa, while it was least on S. aureus. The particles generated at 1 kGy at 1,000 μg/ml concentration showed significantly lower number of colonies of P. aeruginosa against 100 μg/ml. Our study demonstrates that silver nanoparticles generated at 1 kGy irradiation dosage will be of immense value to produce broad spectrum bacteriostatic or bactericidal agents and needs further studies for clinical applications.

Online publication date: Thu, 13-May-2010

 

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