Int. J. of Energy Technology and Policy   »   2015 Vol.11, No.3

 

 

Title: Producton of cellulase by Aspergillus fumigatus JCF using agro-based cellulosic waste and its usage in bioethanol synthesis

 

Authors: Elsa Cherian; R. Kalpana; E. Jayalakshmi

 

Addresses:
Department of Biotechnology, St. Joseph's College of Engineering, Old Mamallapuram Road, Chennai 600119, India
Department of Biotechnology, St. Joseph's College of Engineering, Old Mamallapuram Road, Chennai 600119, India
Department of Biotechnology, St. Joseph's College of Engineering, Old Mamallapuram Road, Chennai 600119, India

 

Abstract: Cellulose is a major polysaccharide in plant cell wall which can be easily be broken down into simpler products by cellulase enzyme. Cellulase is an industrially important enzyme having various applications in industries like textile, paper and pulp, food, brewing, fuel and chemical industry. The relative high cost of cellulase enzyme can be significantly reduced by using agricultural waste as the source of production. In the present study different agricultural wastes like sawdust, corncob and cottonseed were selected for cellulase production using Aspergillus fumigatus JCF. Highest production of cellulase was obtained with cotton seed as the carbon source and peptone as the nitrogen source in submerged fermentation. Optimum conditions for the cellulase production were found to be pH of 6.5 and temperature 50°C. Under optimised conditions maximum cellulase activity was found to be 3.57 IU/ml. Production of biofuel plays an important role in the current era due to its increased demand and price. Cellulase produced at the optimised conditions and baker/s yeast was used for simultaneous saccharification and fermentation for bioethanol production. Maximum bioethanol concentration of 6.7 gm/l was obtained using cotton seed as the substrate at 48 hrs of fermentation.

 

Keywords: cellulase production; Aspergillus fumigatus JCF; baker's yeast; cellulosic waste; bioethanol synthesis; agricultural waste; sawdust; corncob; cotton seed; biofuels; carbon source; peptone; nitrogen source; submerged fermentation.

 

DOI: 10.1504/IJETP.2015.073802

 

Int. J. of Energy Technology and Policy, 2015 Vol.11, No.3, pp.294 - 302

 

Submission date: 13 Mar 2015
Date of acceptance: 14 Jun 2015
Available online: 22 Dec 2015

 

 

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