Title: Multi-layered and conducting polymer-based biosensing membranes

Authors: Yong J. Yuan

Addresses: Crown Research Institutes, Industrial Research Ltd., PO Box 31-310, Lower Hutt, New Zealand

Abstract: The multi-layer membrane designs of enzyme-based electrode have been proven to be a successful approach for biosensing of formate, with substantial improvement in sensitivity and selectivity. The biological interferents can be eliminated by multi-layer designs and hydrodynamic analysis, prevented from oxidising on the gold substrate. Broad linear ranges of ferrocyanide-mediated formate biosensors have been achieved by discrete layered fabrication. The oxidised enzyme or co-factor can be reduced by a highly flexible redox mediator, which cannot diffuse out of the sensor. The use of multi-layer designs for mediated electron transfer can prevent the mediating species from diffusing away from the electrode surface, when the mediating reaction can take place in a volume, rather than at a surface. A more elaborate trilayer system which consists of mediating, biosensing and diffusion-limiting layers has been developed to eliminate biological interferents.

Keywords: polypyrrole; multi-layer biosensing membranes; conducting polymer-based biosensing membranes; polymer-based biosensing membranes; formate biosensors; bioengineering; biomechatronics; enzyme-based electrodes; biological interferents.

DOI: 10.1504/IJISTA.2005.007311

International Journal of Intelligent Systems Technologies and Applications, 2005 Vol.1 No.1/2, pp.126 - 142

Published online: 05 Jul 2005 *

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