Title: SWATH-MS in proteomics: current status

Authors: Jing Wang; Quanhu Sheng; Yu Shyr

Addresses: Center for Quantitative Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA ' Center for Quantitative Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA ' Center for Quantitative Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

Abstract: Sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) is a new strategy for identification and quantification of proteins and proteomes proposed in 2012. SWATH-MS operates in unbiased data-independent acquisition (DIA) mode and combines the advantages of two widely used MS-based techniques: shotgun (high throughput) and SRM proteomics (high sensitivity and reproducibility). In this review, we provide an introduction of the SWATH-MS method, its application, and a perspective on the future of this powerful technology.

Keywords: SWATH-MS; proteomes; sequential window acquisition; fragment ion spectra; tandem mass spectrometry; computational biology; drug design; protein identification; proteome identification; protein quantification; proteome quantification; bioinformatics.

DOI: 10.1504/IJCBDD.2015.072074

International Journal of Computational Biology and Drug Design, 2015 Vol.8 No.3, pp.192 - 202

Received: 23 Aug 2014
Accepted: 18 Oct 2014
Published online: 30 Sep 2015 *

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