Title: Isolation of different viruses from diarrhoeal and non-diarrhoeal stool samples in children at our centre at Karachi, Pakistan

Authors: Q. Mughis Uddin Ahmed; Shin Isomura

Addresses: Karachi Medical and Dental College and ASH, Block-M North, Nazimabad Allama Rasheed Turabi Road, Karachi, Pakistan; Microbiology and Serology Department of Pathology and Laboratory Medicine (105), King Abdulaziz Hospital (NGHA), P.O. Box# 2477, Al-Ahsa-31982, Saudi Arabia ' Department of Zoology and Tropical Medicine, School of Medicine, Nagoya University, 65 Taurumal Cho, Showa-Ku, Nagoya 468, Japan

Abstract: This study was designed to find out different viruses in stool of diarrhoeal and non-diarrhoeal cases in children. This is the first study of its type done at the Abbasi Shaheed Hospital and Karachi-Pakistan. This study is done for period of one year. Stool samples 229 children up to the age of six years were included for isolation of viruses. Rota-virus was excluded from this study (separate study). This study was done in collaboration of the Nagoya University Nagoya, Japan. The 26 cases of clinical polio were subjected for enteroviruses isolation 24 (92.3%) were positive. Diarrhoeal stools were 126 out of which 62 (49.2%) positive for enteroviruses. Stool samples from healthy individuals were 77 from which 17 (22.1%) were having enteroviruses. Overall out of 229 stool samples 103 (45.0%) were having enteroviruses. Different species like polio-1, polio-2, polio-3, non-polio enterovirus, adenovirus (3 and 11), ECHO (3, 6, 7, 12, 19 and 21) and cox A10 were isolated in children. It is necessary for us to look for preventive strategies against these viruses to cope with pathogenic viruses.

Keywords: enteroviruses; polio virus; adenoviruses; ECHO viruses; cox A10 virus; electron microscopy; Pakistan; diarrhoeal stool samples; non-diarrhoeal stool samples; children.

DOI: 10.1504/IJIS.2012.051243

International Journal of Immunological Studies, 2012 Vol.1 No.4, pp.365 - 375

Published online: 30 Aug 2014 *

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