Title: T-cell receptor variable beta (1-24) gene repertoire in patients with Wuchereria bancrofti infections

Authors: M.S. Sudhakar; K.V. Alala Sundaram; R.B. Narayanan

Addresses: Centre for Biotechnology, Anna University, Chennai 600 025, India. ' Department of Plastic Surgery, Royapettah General Hospital, Chennai 600 014, India. ' Centre for Biotechnology, Anna University, Chennai-600 025, India

Abstract: T-cell receptor V beta (TCRV_β) gene repertoire (Vβ1-Vβ24) was evaluated in the peripheral blood mononuclear cells (PBMCs) from asymptomatic and amicrofilaremic normal individuals (EN), and patients with chronic pathology (CP) harbouring Wuchereria bancrofti. TCRV_β gene expression in phytohemagglutinin (PHA) stimulated PBMC cultures from EN and CP individuals was in the order EN > CP while in Brugia malayi adult antigen (BmA) or purified protein derivative from mycobacterium tuberculosis (PPD) stimulation or the unstimulated conditions, the order was CP > EN. Thus, the PBMCs of the CP patients showed elevated levels of TCRV_β gene expression both in the unstimulated and stimulated conditions compared to EN.

Keywords: filariasis; T-cell receptor V beta; TCRV-beta; purified protein derivatives; PPD; Brugia malayi adult antigen; BmA; Wuchereria bancrofti; gene expression; phytohemagglutinin; PHA; peripheral blood cells; blood mononuclear cells; mycobacterium tuberculosis; mycobacterium TB.

DOI: 10.1504/IJMEI.2012.045301

International Journal of Medical Engineering and Informatics, 2012 Vol.4 No.1, pp.25 - 35

Published online: 11 Aug 2014 *

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