Article: Vascular reactivity and metabolism of the reactive forms of oxygen and nitrogen: effects of low doses of radiation Journal: International Journal of Low Radiation (IJLR) 2011 Vol.8 No.2 pp.107 - 121 Abstract: Endothelium-dependent and endothelium-independent vascular reactions of relaxation, the rate of reactive oxygen species (superoxide and hydroxyl) generation, the content of stable nitric oxide (NO) metabolites (nitrite, nitrate and nitrosothiols), the pools of lipid peroxidation products (eicosanoids LTC4 and TxB2, dienic conjugates and malonic dialdehyde), H2O2, uric acid and urea were studied in aorta preparations of BALB/c 9 months-old mice: group I – control; group II – exposed to chronic external low doses ?-irradiation (equivalent dose of 96.9 ?Sv h?1) mice (cumulative dose of 0.43 Sv). The results obtained showed disturbances in the studied reactions and change of the reactive forms of oxygen and nitrogen following an effect of low doses of radiation. The latter induce significant changes in the pools of NO stable metabolites, which can cause disturbances in the NO-dependent physiological functions of heart and aorta. A significant decrease in the levels of nitrite and S-nitrosothiols can result in nitrosative stress. Inderscience Publishers - linking academia, business and industry through research

Title: Vascular reactivity and metabolism of the reactive forms of oxygen and nitrogen: effects of low doses of radiation

Authors: Mikhail N. Tkachenko; Anatolii V. Kotsjuruba; Olga V. Bazilyuk; Irina V. Gorot; Oleg I. Remennik; Vadim F. Sagach

Addresses: A.A. Bogomoletz National Medical University, 13, Shevchenko Blvd., Kiev 01601, Ukraine. ' A.V. Palladin Institute of Biochemistry NAS Ukraine, 9, Leontovich St., Kiev 01601, Ukraine. ' Bogomoletz Institute of Physiology, 4, Bogomoletz St., Kiev 01024, Ukraine. ' A.A. Bogomoletz National Medical University, 13, Shevchenko Blvd., Kiev 01601, Ukraine. ' Kyiv Oblast Oncology Dispanser, 1, Baggovutivska St., 04107 Kiev, Ukraine. ' Bogomoletz Institute of Physiology, 4, Bogomoletz St., Kiev 01024, Ukraine

Abstract: Endothelium-dependent and endothelium-independent vascular reactions of relaxation, the rate of reactive oxygen species (superoxide and hydroxyl) generation, the content of stable nitric oxide (NO) metabolites (nitrite, nitrate and nitrosothiols), the pools of lipid peroxidation products (eicosanoids LTC₄ and TxB₂, dienic conjugates and malonic dialdehyde), H₂O₂, uric acid and urea were studied in aorta preparations of BALB/c 9 months-old mice: group I – control; group II – exposed to chronic external low doses ?-irradiation (equivalent dose of 96.9 ?Sv h^?1) mice (cumulative dose of 0.43 Sv). The results obtained showed disturbances in the studied reactions and change of the reactive forms of oxygen and nitrogen following an effect of low doses of radiation. The latter induce significant changes in the pools of NO stable metabolites, which can cause disturbances in the NO-dependent physiological functions of heart and aorta. A significant decrease in the levels of nitrite and S-nitrosothiols can result in nitrosative stress.

Keywords: BALB/c mice; chronic external gamma-irradiation; endothelium dysfunction; low dose irradiation; low radiation; nitrate; nitrite; S-nitrosothiols; oxygen reactive forms; vascular reactivity; metabolism; nitrogen; aorta preparations; heart functions.

DOI: 10.1504/IJLR.2011.044192

International Journal of Low Radiation, 2011 Vol.8 No.2, pp.107 - 121

Published online: 07 Feb 2015 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article

Title: Vascular reactivity and metabolism of the reactive forms of oxygen and nitrogen: effects of low doses of radiation

Keep up-to-date