Int. J. of Nano and Biomaterials   »   2011 Vol.3, No.3

 

 

Title: In vitro and in vivo evaluation of an injectable premixed calcium phosphate cement; cell viability and immunological response from rat

 

Author: J. Aberg, H.B. Henriksson, H. Engqvist, A. Palmquist, A. Lindahl, P. Thomsen, H. Brisby

 

Addresses:
Applied Materials Science, Department of Engineering Sciences, Uppsala University, Regementsvagen 1, 752 37 Uppsala, Sweden.
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, 413 45, Gothenburg, Sweden.
Applied Materials Science, Department of Engineering Sciences, Uppsala University, Regementsvagen 1, 752 37 Uppsala, Sweden.
Department of Biomaterials, Institute for Clinical Sciences, Gothenburg University, 413 45, Gothenburg, Sweden.
Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, 413 45, Gothenburg, Sweden.
Department of Biomaterials, Institute for Clinical Sciences, Gothenburg University, 413 45, Gothenburg, Sweden.
Department of Orthopaedics, Sahlgrenska University Hospital, Gothenburg University, 413 45 Gothenburg, Sweden

 

Abstract: By using premixed calcium phosphate cement (CPC) the handling properties of the cement are drastically improved, which is a challenge for traditional injectable CPC. In this article, a premixed acidic CPC has been compared to a conventional water mixed brushite cement to evaluate whether the premixed concept affects the biological response. The cements were evaluated regarding the pH-variation in simulated body fluid (SBF). Further, the biocompatibility of the cement with human mesenchymal stem cells (MSC) was studied in vitro and eventual inflammation properties studied in vivo, after subcutaneous material injections in rats. A larger pH decrease was seen for the conventional cement than the premixed cement in SBF. For both materials, > 90% of the MSC remained alive in vitro. No systemic or macroscopic inflammation was detected, only a mild microscopic inflammation was detected around both materials. In addition to the handling benefit of premixed cements compared to conventional water mixed CPC, the premixed CPC in the present study demonstrated high and in comparison to conventional CPC comparable biocompability, both in vitro and in vivo.

 

Keywords: premixed CPC; in vitro evaluation; in vivo evaluation; rats; injectable CPC; mesenchymal stem cells; MSC; calcium phosphate cement; immunological response; brushite; monetite; cell viability; biocompatibility; pH variation; simulated body fluid; inflammation; premixed cements.

 

DOI: 10.1504/IJNBM.2011.042130

 

Int. J. of Nano and Biomaterials, 2011 Vol.3, No.3, pp.203 - 221

 

Submission date: 08 Apr 2010
Date of acceptance: 30 Oct 2010
Available online: 28 Aug 2011

 

 

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