Title: Simultaneous or sequential administration of botulinum neurotoxin E does not reduce the duration of paralysis caused by botulinum neurotoxin A in rat EDL muscle

Authors: Michael Adler, Sharad S. Deshpande, James P. Apland

Addresses: Neurobehavioral Toxicology Branch, Analytical Toxicology Division, US Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, MD 21010-5400, USA. ' Neurobehavioral Toxicology Branch, Analytical Toxicology Division, US Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, MD 21010-5400, USA. ' Neurobehavioral Toxicology Branch, Analytical Toxicology Division, US Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground, MD 21010-5400, USA

Abstract: Exposure to botulinum neurotoxin serotype A (BoNT/A) or serotype E (BoNT/E) results in muscle paralysis owing to cleavage of the SNARE protein SNAP-25 between residues 197-198 or 180-181, respectively. Although SNAP-25 is the substrate for both serotypes, the duration of paralysis is considerably longer for muscles exposed to BoNT/A. This study was designed to determine whether paralysis times were determined by differences in the half-lives of the truncated SNAP-25 fragments or by differences in the persistence of the respective BoNT light chain (LC) within the nerve terminals.

Keywords: botulinum neurotoxin; BoNT/A; BoNT/E; persistence; rats; EDL muscle; paralysis; recovery; twitch tension; tetanic tension; BoNT; botulism; SNAP-25; nerve terminals; nerve ends.

DOI: 10.1504/TBJ.2010.039118

The Botulinum Journal, 2010 Vol.1 No.4, pp.442 - 456

Published online: 17 Mar 2011 *

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