Title: Acute toxicity of three antifungal chemicals on silverside Odontesthes bonariensis (Valenciennes 1835) eggs

Authors: Suani Giovanna Pacheco-Marino, Alfredo Salibian

Addresses: CONICET, Rivadavia 1917, C1033AAJ-Buenos Aires, Argentina; Instituto de Botanica Carlos Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina. ' CIC Provincia de Buenos Aires, La Plata, Argentina; Departamento de Ciencias Basicas (PRODEA-INEDES), Universidad Nacional de Lujan, P.O. Box 221, (B6700ZBA)-Lujan, Argentina

Abstract: The effects of sodium chloride (NaCl) and formalin (F) on some toxicological parameters and the hatching rate of Odontesthes bonariensis eggs were examined under laboratory conditions, and the determined values were compared with those obtained for Malachite Green (MG), which was used as a reference toxic. The 96 hours nominal LC₅₀ were 1.51 mg L⁻¹, 652.14 μl L⁻¹ and 34.82 g L⁻¹ for MG, F and NaCl, respectively. The No-Observed-Effect Concentration (NOEC) and the No-Effect Concentrations (NEC) were 1.5 mg L⁻¹ and 0.507 mg L⁻¹ (MG), 400 μl L⁻¹ and 530 μl L⁻¹ (F) and 30.0 g L⁻¹ and 28.68 g L⁻¹ (NaCl), respectively. Hatching rates of 60% or higher were determined for eggs after 168 hours incubation in the assayed solutions at concentrations of 0.75 mg L⁻¹ (MG), 300 μ L⁻¹ (F) and 20 g L⁻¹ (NaCl); at higher concentrations the hatching rates were severely affected.

Keywords: Odontesthes bonariensis; fish eggs; fungicides; NOEC; no-observed-effect concentration; NEC; no-effect concentrations; hatching rate; acute toxicity; antifungal chemicals; sodium chloride; formalin.

DOI: 10.1504/IJENVH.2010.037498

International Journal of Environment and Health, 2010 Vol.4 No.4, pp.333 - 341

Published online: 14 Dec 2010 *

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