Title: Distinct role of non-covalent interactions to the function and structural stability of glutaredoxins: a multifunctional redox protein

Authors: Vibhu Ranjan Prasad, Bhumi Nath Tripathi, Rao Sethumadhavan

Addresses: School of Biotechnology, Chemical and Biomedical Engineering, Vellore Institute of Technology, Vellore, 632 014 Tamil Nadu, India. ' Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304 022 Rajasthan, India. ' School of Biotechnology, Chemical and Biomedical Engineering, Vellore Institute of Technology, Vellore, 632 014 Tamil Nadu, India

Abstract: In this work, the role of cation-π and CH…π interactions in the activity of glutaredoxins is analysed. Among the proteins, an average of 1 cation-π interaction per 109 residues and an average of 1 CH…π interaction per 16 residues were found. These interactions were influenced by long-range contacts whereas short- and medium-range contacts were found insignificant. Significant differences in these interactions were noticed when the same glutaredoxin was analysed in its glutathionylated, reduced and oxidised states. Since activities of glutaredoxins depend on its state, the role of these interactions in regulation of these proteins might be significant.

Keywords: glutaredoxins; cation-pi; non-covalent interactions; CH...pi interaction; long-range contacts; redox proteins; function; structural stability; residues; bioinformatics.

DOI: 10.1504/IJBRA.2010.034073

International Journal of Bioinformatics Research and Applications, 2010 Vol.6 No.3, pp.241 - 259

Received: 13 Jul 2009
Accepted: 28 Sep 2009

Published online: 07 Jul 2010 *

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