Title: L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility

Authors: Raymond Wing Moon Lam, Chi Tak Wong, Zhao Yang Li, Keith Dip Kei Luk, Kenneth M.C. Cheung, Peter Kwong Yuen Chiu, Bing Xu, Wai Kin Chan, David Fang, John C.Y. Leong, William Weijia Lu

Addresses: Faculty of Medicine Building, Department of Orthopaedics and Traumatology, The University of Hong Kong, Rm908, Lab Block, 21 Sassoon Road, Pokfulam, Hong Kong. ' Faculty of Medicine Building, Department of Orthopaedics and Traumatology, The University of Hong Kong, L912 Lab Block, 21 Sassoon Road, Pokfulam, Hong Kong. ' Faculty of Medicine Building, Department of Orthopaedics and Traumatology, The University of Hong Kong, L906 Lab Block, 21 Sassoon Road, Pokfulam, Hong Kong. ' Department of Orthopaedics and Traumatology, The University of Hong Kong, 5/F Professor Building, Queen Mary Hospital, Hong Kong. ' Department of Orthopaedics and Traumatology, The University of Hong Kong, 5/F Professor Building, Queen Mary Hospital, Hong Kong. ' Department of Orthopaedics and Traumatology, The University of Hong Kong, Room 216, New Clinical Building, Queen Mary Hospital, Pokfulam, Hong Kong. ' Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China. ' Department of Chemistry, The University of Hong Kong, Room 403, Chong Yuet Ming Chemistry Building, Pokfulam Road, Hong Kong. ' Jointec Ltd., Rm 1118, Central Golf Bank Of America Tower, 12 Harcourt Road, Central, Hong Kong, China. ' The Open University of Hong Kong, 30 Good Shepherd Street, Ho Man Tin, Kowloon, Hong Kong, China. ' Faculty of Medicine Building, Department of Orthopaedics and Traumatology, The University of Hong Kong, Rm907, Lab Block, 21 Sassoon Road, Pokfulam, Hong Kong

Abstract: In this study, we investigated the influence of L-Asparagine (L-Asn) on strontium hydroxyapatite (Sr-HA) nanorod morphology and their biocompatibility. Ammonia was used to adjust the pH of L-Asn Sr-HA precursor and was heated at 80-120°C for 5-15 h. From Transmission Electron Microscopy|s (TEM) images, the lengths and diameters of the nanorod were 0.3901 ± 0.0804 µm and 30.4 ± 4.6 nm, respectively. L-Asn Sr-HA phosphate system provides a high phase purity of monodispersed strontium hydroxyapatite nanorod. From Live/Dead Assay, L-Asn Sr-HA nanorod showed cell-dependent cytotoxicity (SaOS-2 < C2C12 < L929) and these results have shown L-Asn Sr-HA nanorod can be adopted as fibrous tissue formation inhibitor.

Keywords: amino acid; strontium hydroxyapatite nanorods; cytotoxicity; L-Asparagine; morphology; biocompatibility; nanotechnology; bioactive bone cement; vertebroplasty bone cement.

DOI: 10.1504/IJBET.2009.027795

International Journal of Biomedical Engineering and Technology, 2009 Vol.2 No.4, pp.303 - 318

Published online: 11 Aug 2009 *

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