Title: A strategy for Ordered single molecule Sequencing based on Nanomanipulation (OsmSN)

Authors: Yi Zhang, Junhong Lu, Minqian Li, Jun Hu

Addresses: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P.R. China. ' Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P.R. China. ' Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P.R. China. ' Shanghai Institute of Applied Physics, Chinese Academy of Sciences, P.O. Box 800-204, Shanghai 201800, P.R. China; Bio-X Life Science Research Center, College of Life Science and Biotechnology, Shanghai JiaoTong University, Shanghai 200030, China

Abstract: In this mini-review we present the concept and the advancement of a novel strategy termed ||ordered single molecule sequencing based on nanomanipulation (OsmSN)|| for whole-genome sequencing. Techniques have been developed to integrate the processes including dissection and isolation of DNA molecules with sufficient spatial resolution together with the subsequent single molecule PCR to achieve the analysis of a particular DNA fragment from a genome DNA molecule. A nanoparticle-assisted PCR protocol was also developed to enhance the specificity and yield of PCR to meet the special requirement of the OsmSN.

Keywords: DNA sequencing; ordered single molecule sequencing; nanomanipulation; OsmSN; atomic force microscopy; AFM; nanodissection; isolation; single-molecule PCR; polymerase chain reaction; nanoparticle PCR; nanoparticles; nanotechnology.

DOI: 10.1504/IJNT.2007.012321

International Journal of Nanotechnology, 2007 Vol.4 No.1/2, pp.163 - 170

Published online: 05 Feb 2007 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article