In-situ ellipsometric study of calcium phosphate biomineralisation on organic thin films
by Rayomand Shahlori; Geoffrey I.N. Waterhouse; Andrew R.J. Nelson; Duncan J. McGillivray
International Journal of Nanotechnology (IJNT), Vol. 14, No. 1/2/3/4/5/6, 2017

Abstract: In this study, in-situ ellipsometry and scanning electron microscopy (SEM) were used to follow the growth kinetics of calcium phosphate (CaP) films on zein and hexadecanoic acid (HDA) layers self-assembled at the air-liquid interface. The duration of the mineral film induction period was heavily dependent on the organic template used, presence or absence of Mg²⁺, solution pH and concentration. In a standard simulated body fluid (SBF) solution at 20°C and pH 7.4, CaP film growth occurred after an induction period of 40 min for HDA and 100 min for zein. In the absence of Mg²⁺ the mineralisation induction period was reduced, for only the zein protein, to 50 min. At pH 6, HDA and zein-induced CaP films started forming after 100 min and 180 min, respectively. No induction period was observed when the SBF concentration was doubled, with immediate CaP growth observed for both HDA and zein. This study shows the effect of biomineralisation conditions on the growth of CaP with the organic template playing an important role. Knowledge of the factors that influence mineral growth processes is of importance in areas of biomineralisation and controlled crystal growth.

Online publication date: Fri, 24-Feb-2017

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