Comparison investigation of several hydrogen peroxide biosensor based on the direct electrochemistry of horseradish peroxidase immobilised on self-assembled monolayers Online publication date: Sat, 17-May-2014
by Chuanyin Liu
International Journal of Nanomanufacturing (IJNM), Vol. 10, No. 1/2, 2014
Abstract: Horseradish peroxidase was immobilised onto self-assembled monolayers (SAMs) by electrostatic adsorption, acylimide and Sciff base covalent-linkage, respectively. The effects of different immobilised methods on the direct electrochemistry of HRP and electrocatalysis towards H2O2 have been evaluated. The experimental results reveal that the direct electrochemistry of HRP can be achieved by the method of acylimide linkage. The electron transfer coefficient α and rate constant ks have been calculated to be 0.70 and 33.88 s-1 for HRP-GSH/Au, 0.52 and 11.35 s-1 for HRP-Cys/Au, respectively. The response is linear to H2O2 concentration from 1 µM to 1.4 mM and lower detection limit of 0.46 µM for HRP-GSH/Au, 1.5µM to 1.2 mM and lower detection limit of 0.76 µM for HRP-Cys/Au. The biosensor showed longer stability and favourable reproducibility and anti-interfering ability.
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