Article Abstract
| Title: | Utility of Botulinum Toxin ELISA A, B, E, F kits for clinical laboratory investigations of human botulism | |
| Author: | Susan E. Maslanka, Carolina Luquez, Brian H. Raphael, Janet K. Dykes, Lavin A. Joseph 
|
|
| Address: | National Botulism Laboratory Preparedness Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. ' National Botulism Laboratory Preparedness Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. ' National Botulism Laboratory Preparedness Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. ' National Botulism Laboratory Preparedness Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA. ' National Botulism Laboratory Preparedness Team, Enteric Diseases Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA | |
| Journal: | The Botulinum J. 2011 - Vol. 2, No.1 pp. 72 - 92 | |
| Abstract: | The Botulinum Toxin ELISA effectively provided presumptive identification of toxin in 1381 investigation samples including clinical specimens, suspect foods, and cultures. Additionally, the ELISA detected all toxins produced by a panel of stock strains representing known subtypes and was negative for non-botulinum toxin producing Clostridium and enteric pathogens. ELISA results were reproducible both within the same kit (CV < 9%) and among different production lots (CV < 23%). Fifty-five of 57 laboratories correctly identified unknown samples in a multi-laboratory study. The ELISA provides a rapid, robust in vitro screening method which will reduce animal dependence during laboratory investigations of botulism. | |
| Keywords: | botulinum toxin ELISA; foods; clinical specimens; cultures; human botulism; in vitro botulinum detection; ELISA performance optimisation; botulinum toxin subtypes; diagnostic antibody production; mouse bioassay. | |
| DOI: | 10.1504/TBJ.2011.041817 | |
Purchase this article  Comment on the article |
||